Optimal Target Prediction and Visualization for Genome Editing with CRISPR-Cas Systems

CRISPR-DT is a web application that allows a user to upload DNA sequences, set specifications according to experimental goals, and recieve target candidates. Optimal candidates are suggested through consideration of predicted target efficiency and off-target effect. A visualization of on- and off-target matches against the chosen reference genome is provided in JBrowse.

Four modes are available:

(1) Cpf1 system - Returns single target candidates

(2) Cas9 system - Returns single target candidates

(3) Cas9 nickase (Cas9n) system - Returns paired target candidates

(4) RNA guided Fok1 nuclease (RFN) system - Returns paired target candidates



How to Cite: Houxiang Zhu, Chun Liang, CRISPR-DT: designing gRNAs for the CRISPR-Cpf1 system with improved target efficiency and specificity. Bioinformatics doi:10.1093/bioinformatics/bty1061

Our web application for RNA Targeting with the CRISPR-C2c2/Cas13a system is available here.