RNA-Targeting Prediction and Visualization with the CRISPR-C2c2/Cas13a System
CRISPR-RT is a web application that allows a user to upload an RNA sequence, set specifications according to experimental goals, and recieve target candidates for the CRISPR-C2c2 System (now called CRISPR-Cas13a). Optimal candidates are suggested through consideration of predicted off-target effects. A visualization of on- and off-target matches against the chosen reference transcriptome is provided in JBrowse.
Open Source: https://github.com/bioinfolabmu/CRISPR-RT
How to Cite: Zhu, H., Richmond, E. & Liang, C. CRISPR-RT: A web application for designing CRISPR-C2c2 crRNA with improved target specificity. Bioinformatics doi:10.1093/bioinformatics/btx580
Our web application for DNA Targeting with CRISPR systems is available here.